2 edition of Changes in stability of transposable elements in Antirrhinum majus. found in the catalog.
Changes in stability of transposable elements in Antirrhinum majus.
Andrew David Hudson
Thesis (Ph.D.), University of East Anglia, School of Biological Sciences, 1987.
Transposon insertions occasionally occur in the promoter regions of plant genes, many of which are still capable of being transcribed. However, it remains unclear how transcription of such promoters is able to occur. Insertion of the Tam3 transposon into various genes of Antirrhinum majus can confer leaky phenotypes without its excision. These genes, named Tam3 . The snapdragon (Antirrhinum majus) centroradialis mutant (cen) is characterized by the development of a terminal flower, thereby replacing the normally open inflorescence by a closed inflorescence. In contrast to its Arabidopsis counterpart, terminal flower1, the cen -null mutant displays an almost constant number of lateral flowers below the terminal flower.
Resistance to gap repair of the transposon Tam3 in Antirrhinum majus: A role of the end regions Article (PDF Available) in Genetics (4) January with 16 Reads How we measure 'reads'. Insertions of a Tam3 element into the first intron of the plena locus in Antirrhinum majus have opposite effects depending on the orientation of the transposon. In one orientation the result of an insertion is a recessive homeotic conversion of sex organs to sterile perianth organs because of loss of expression in the inner two whorls of the flower.
We present the genomic structure of Tam1, a transposable element from Antirrhinum majus. The Taml element is kb long and includes two genes that are transcribed to produce a . Coen ES, Carpenter R, Martin C (): Transposable elements generate novel spatial patterns of gene expression in Antirrhinum majus. Cell – Coen ES, Carpenter R (): A semi‐dominant allele, niv‐, acts in trans to inhibit expression of its wild‐type homologue in Antirrhinum majus.
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Tam3 in Antirrhinum majus is exceptional among cut-and-paste-type transposons (TEs) in that it is the only known TE whose transpositional behavior can be strictly controlled by environmental influence. Tam3 transposition is strongly affected by temperature: It is active at low temperatures (around 15°C) and stable at high temperatures (around 25°C; Harrison and Cited by: Transposable Element Terminal Inverted Repeat Genomic Southern Blot Antirrhinum MAJUS Chimeric mRNA These keywords were added by machine and not by the authors.
This process is experimental and the keywords may be updated as the learning algorithm by: Transpos- able elements have now been isolated from several prokaryotes and eukaryotes and shown to be segments of DNA (usually bp) normally having inverted Transposable elements in Antirrhinum majus: generators of genetic diversity Enrico S.
Coen and Rosemary Carpenter Several unstable mutations in AnErrl~num majus are caused by Cited by: Abstract. We describe the structure of a chromosome rearrangement which changes the spatial pattern of expression of the pallida gene of Antirrhinum rearrangement involves a chromosome inversion of ~6 map units with one breakpoint at the end of a copy of the transposable element Tam3 located in the promoter region of the pallida locus.
The Cited by: Changes in stability of transposable elements in Antirrhinum majus Author: Hudson, A. ISNI: Awarding Body: University of East Anglia Current Institution: University of East Anglia Date of Award: Availability of Full Text.
The pallida gene of A. majus encodes a product required for the synthesis of red flower pigment. We have shown that the unstable pallida recurrens mutation is due to the insertion of the Tam3 transposable element near the promoter of the gene.
Imprecise excision of Tam3 alters pallida gene expression and generates new spatial patterns or different intensities of. Coen, E. S., Carpenter, R. & Martin, C.
Transposable elements generate novel spatial patterns of gene expression in Antirrhinum majus. C – (). Article CAS PubMed Google Scholar. TAM are responsible for the high mutability of genes controlling the synthesis of flower pigments, known in this plant since pre-Mendelian times.
There are several TAM elements. The termini of the TAM1 and TAM2 are homologous and their insertion results in a 3 bp target site termini are almost identical to those of the Spm/En transposons of maize and.
Summary. The kb transposable element, Tam3, has been shown to cause somatic and germinal instability at the nivea locus, which encodes chalcone synthase, of Antirrhinum lar cloning and sequence analysis of the nivTam3 allele revealed that the termini of Tam3 consist of 12 bp perfect inverted 3 is integrated in the promoter.
Chomet PS, Wessler S, Dellaporta SL. Inactivation of the maize transposable element Activator (Ac) is associated with its DNA modification. EMBO J. Feb; 6 (2)– [Europe PMC free article] [Google Scholar] Coen ES, Carpenter R, Martin C. Transposable elements generate novel spatial patterns of gene expression in Antirrhinum majus.
A special challenge for variety fingerprinting arises in ornamentals sold as population mixture or F1 hybrids displaying colour combinations. We developed a fingerprint protocol for snapdragon (Antirrhinum spp.) commercial plant material based on mapped miniature inverted-repeat transposable used 15 MITEs-based markers to.
It has been proposed that environmental stimuli can activate transposable elements (TEs), whereas few substantial mechanisms have been shown so far. The class‐II element Tam3 from Antirrhinum majus exhibits a unique property of low‐temperature‐dependent transposition (LTDT).
LTDT has proved invaluable in developing the gene isolation. The discovery and genetic characterization of plant transposable elements (TEs) led to a revolution in our understanding of the composition and dynamic potential of the genetic material in virtually all organisms.
Most of these breakthroughs occurred between 30. Lister and C. Martin John Innes Institute and AFRC Institute of Plant Sciences Research, Norwich, NR4 7UH, England The transposable element Tam3 of Antirrhinum majus is capable of causing large-scale chromosomal restructuring. It induced a large deletion at the nivea locus, to produce the allele niv(-) The deletion removed the entire nivea coding region while the element.
Martin C, Mackay S, Carpenter R. Large-scale chromosomal restructuring is induced by the transposable element tam3 at the nivea locus of antirrhinum majus. Genetics. May; (1)– [PMC free article] Masson P, Surosky R, Kingsbury JA, Fedoroff NV.
Genetic and molecular analysis of the Spm-dependent a-m2 alleles of the maize a locus. The Antirrhinum majus transposon Tam3 undergoes low temperature–dependent transposition (LTDT).
Growth at 15°C permits transposition, whereas growth at 25°C strongly suppresses it. The degree of Tam3 DNA methylation is altered somatically and positively correlated with growth temperature, an exceptional epigenetic system in plants.
Using a Tam3-inactive line, we show. transposable elements causing unstable mutations in E. coil were found and physically characterized. Transpos- able elements have now been isolated from several prokaryotes and eukaryotes and shown to be segments of DNA (usually bp) normally having inverted Transposable elements in Antirrhinum majus: generators.
Transposable elements ([TE]s) are considered to be parasites of host genomes because they act as powerful mutagens. If not kept in check, they can cause gene disruption, genome rearrangement, and genomic takeover.
Hence, activities of [TE]s are under the rigid control of hosts. To date, all identified [TE] regulations have been epigenetic dependent, with the. Antirrhinum majus. Quotation marks indicate the change of floral primordium development in the corresponding mutant.
W, whorl. Antirrhinum. These transposable elements can be. The transposable element Tam3 of Antirrhinum majus generates a novel type of sequence alterations upon excision. Mol Gen Genet. ; – Tröbner W, Ramirez L, Motte P, Hue I, Huijser P, Lönnig WE, Saedler H, Sommer H, Schwarz-Sommer Z.
GLOBOSA: a homeotic gene which interacts with DEFICIENS in the control of Antirrhinum floral. The extremely homogeneous organization of the transposon family Tam3 in Antirrhinum majus is in sharp contrast to the heterogeneity of the copies constituting many other transposon families.
To address the issue of the Tam3 structural uniformity, we examined two possibilities: (1) recent invasion of Tam3 and (2) failure of gap repair, which is involved in conversion from. Coen ES, Carpenter R, Martin C.
Transposable elements generate novel spatial patterns of gene expression in Antirrhinum majus. Cell. Oct 24; 47 (2)– Coen ES, Romero JM, Doyle S, Elliott R, Murphy G, Carpenter R. floricaula: a homeotic gene required for flower development in antirrhinum majus.
Cell. Dec 21; 63 (6)–Two novel ways in which changes in gene expression in A. majus arise as a consequence of the Tam3 transposition mechanism are described.
One involved excision of Tam3 from the nivea gene promoter and insertion of 2 new Tam3 copies kb and kb away, on either side of the excision site. One of the new insertions was in the nivea coding region and completely blocked .